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PPI-HotSpotDB and PPI-HotSpot+PDBBM
A database and benchmark set of Protein-Protein Interaction Hotspots.

Chen Yao-Chi1, Chen Yu-Hsien2, Jon D. Wright1 and Carmay Lim1,2

1Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
2Department of Chemistry, National TsingHua University, HsinChu, Taiwan

This server contains a curated database of residue mutations that are known to disrupt protein-protein interactions.

Submission area choose option A or Option B:

For your target protein provide either a PDB code or a UniProt code. An example of the results for the uniprot O04197 code can be found on this page.

Option A) For a known PDB 4-letter code.
Provide a PDB code:

Option B) For a UniProt code.
Paste your UniProt code:


You can download the entire PPI-HotSpotDB and the associated PPI-HotSpot+PDBBM from this page.


NCBI, Resource Coordinators. 2017. 'Database Resources of the National Center for Biotechnology Information.', Nuc. Acids Res., 45: D12–D17. DOI:

UniProt, Consortium. 2021. 'UniProt: the universal protein knowledgebase in 2021.', Nuc. Acids Res., 49: D480-D89. DOI:

H.M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T.N. Bhat, H. Weissig, I.N. Shindyalov, P.E. Bourne. (2000) The Protein Data Bank Nuc. Acids Res., 28: 235-242. DOI:

This server was created by Chen Yao-Chi , Chen Yu-Hsien, Jon D Wright and Carmay Lim and is hosted at The Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan.